Children of early and preschool age with chronic diseases are faced with fears that include separation from their parents and various medical procedures. No matter what chronic illness the child has, it must have the right to be integrated into regular kindergarten programs. This important role is not only parents, but also preschool teachers and peers in the preschool group. Preschool teachers must have certain knowledge and skills related to chronic diseases and the interventions they require because they are often the first to react to certain seizures or complications. One of the most common diseases in preschool children is diabetes. Diabetes or diabetes mellitus type I is the most common form of diabetes occurring in children. It is because of the inability of the pancreas to produce insulin. Insulin dependency is absolute and diet is not enough. Treatment of diabetes is complex. A child must be given a daily dose of insulin whose units are determined according to his age and body mass. The aim of this research is to establish the level of awareness of the preschool teachers in working with children of preschool age with diabetes. A measurement instrument which was used in this research is questionnaire. It was applied to a sample consisting of 39 students of the 3rd year of the Early Childhood and Preschool education in Osijek and 40 kindergarten teachers in Osijek and Đakovo. The questionnaire consists of 3 questions related to age, profession and years of employment and 15 statements that offered a scale from 1 to 4, with students and educators performing self-assessment of their knowledge and experience. The results show that educators are better acquainted with diabetes type I than students but not so widely. The hypothesis that the educators have the appropriate knowledge about diabetes type I and the interventions needed is not confirmed. The results confirm the fact that more and more education is needed among all of us who work or will work with such children in kindergartens.