Musical abilities are the result of hereditary factors and informally and formally acquired knowledge, primarily related to the possibility of successful performance and mastering of a specific musical task, and also develop in a stimulating environment that contributes to the creation of an aesthetic relationship to art. In kindergarten they become more developed and stronger when they are carried out through daily musical activities. Regular participation in musical activities also encourages creativity, confidence and teamwork, while providing children with satisfaction and emotional expression. The musical activity of singing songs is most prevalent in work with children of preschool age. Considering its representation in work with children of preschool age, it contributes to the development of musical abilities: hearing, rhythm, memory, voice culture and singing skills. The musical activity of singing is most often carried out with the instrumental accompaniment of the piano, but the action research described in this paper confirmed that musical activities can also be carried out using an alternative instrument, tambura, and that children are very happy to accept tambura as an instrument when performing musical activities. Tambura, as a folk string instrument, offers the opportunity for children to acquire knowledge about cultural heritage and tradition, and at the same time develop a love for music.