Preschool conflict is a frequent and unavoidable phenomenon which, if approached in an adequate way, can encourage the development of a child's social skills. No child is too young to learn to cooperate with others and resolve conflicts. Conflict is defined as the interaction of two or more people categorized as a mismatch between goals, desires, behaviors, and needs. Therefore, constructive conflict resolution is an essential social skill, which includes having the ability to actively listen, express feelings, needs, desires and seek various possible solutions. Mediation is one of the most important ways of non-violent conflict resolution because it leads to a real acceptance of needs and interests and an effective and long-term resolution of the conflict. The aim of this research is to, through the analysis and synthesis of scientific knowledge about conflict and mediation, identify, describe and evaluate the possibilities of applying mediation in education and communication with preschool children. The results of the research indicate that mediation in preschool institutions is possible and, in most cases, should be conducted by preschool teachers in conflicts between children. Mediation also provides children with insight into constructive conflict resolution, a sense that they are heard and understood, gives them opportunity to take someone else's perspective, look for the best solutions and cooperate with each other.